Transformation of DHN1 gene and DHN promoter constructs into sugarcane calli, regeneration of the calli, and acclimatization of the plantlets

Dehydrin is known to have an important role in plant response and adaptation abiotic stresses including drought high salinity. Previous research reported the isolation of full-length coding sequence (CDS) DHN1 from sugarcane var. PSJT 941, it shares a homology with DHN genes sorghum other varieties. In this study, CDS was cloned under constitutive CaMV35S promoter transformed into calli mediated by Agrobacterium tumefaciens. The promoter, Pr-1DHNSo, also successfully isolated 941 pBI121 expression vector. construct subsequently Kidang Kencana. Transgenic carrying gene constructs were regenerated according standard protocol tissue culture. Optimization acclimatization using modified post-rooting media conducted resulting reduced total mortality rates plantlets. presence periodically tested PCR specific primers. genotyping results showed that present for more than year after transformation.